Platform Technologies and Services Catalogue

To access any of these services please contact Reno Pontarollo

1. Mutant Production
Table A - Resources for Generating Genetically Altered Mice
Table B - Services for Generating Genetically Altered Mice

2. Phenotyping
Table C - Services for Phenotyping Genetically Altered Mice

3. Genotyping & Gene Expression Analysis
Table D - Services for Genotyping & Gene Expression of Genetically Altered Mice

4. Archiving, Maintenance, Recovery & Distribution
Table E - Services for Archiving, Maintenance, Recovery & Distribution of Genetically Altered Mice

5. Informatics & Databases
Table F - Services for Bioinformatics and Database Resources of Genetically Altered Mice

 


1. Mutant Production

Table A - Resources For Generating Genetically Altered Mice
Service Description Estimated Throughput Source
ES Cell Lines
To access services below please contact Reno Pontarollo
Inbred R1 cells (129X1 x 129S1)   Nagy Lab
Inbred C2 cells (C57BL/6)   Nagy Lab
Inbred D3H cells (129S2)   MFGC
Hybrid G4 cells (129S6 x C57BL/6 - F1)   Nagy Lab
Pre-Designed/ Modified EGFP R1 clones: B5 and D4 (X-linked)   Nagy Lab
Pre-Designed/ Modified EYFP R1 clones: 7AC5 and YC5   Nagy Lab
Pre-Designed/ Modified ECFP R1 clones: CK6 and CK8   Nagy Lab
Pre-Designed/ Modified Hybrid EYFP line YV1 (C57BL/6 x 7AC5/EYFP-129S1)   Nagy Lab
Pre-Designed/ Modified Z/RED and dsRED clones of G4   Nagy Lab
pMagic ptac-MBP-H3-Phe-H4-HA3 recipient   Hughes Lab
pMagic ptac-MBP-H3-Phe-myc3 recipient   Hughes Lab
pMagic ptac-MBP-H3-Phe-his10 recipient   Hughes Lab
pMagic ptac-MBP-H3-Phe-stop recipient   Hughes Lab
pMagic T7-GST-H3-Phe-H4-HA3 recipient   Hughes Lab
pMagic T7-GST-H3-Phe-myc3 recipient   Hughes Lab
pMagic T7-GST-H3-Phe-his10 recipient   Hughes Lab
pMagic T7-GST-H3-Phe-stop recipient   Hughes Lab
Vectors
To access services below please contact Reno Pontarollo
Targeting pPNT loxP neo   Rossant Lab
RNAi Ras Gap siRNA   Rossant Lab
Others pCAGGS NLS Cre   Nagy Lab
Others pCAGGS EYFP   Nagy Lab
Others pCAGGS EGFP   Nagy Lab
Others pCAGGS ECFP   Nagy Lab
Others pCAGGS dsRED   Nagy Lab
Others pCCALL2   Nagy Lab
Others pZ/AP   Nagy Lab
Others pZ/EG   Nagy Lab
Others pKS attP/attP   Nagy Lab
Others pKS attB/attB   Nagy Lab
Libraries
To access services below please contact Reno Pontarollo
Genomic RPCI-22 Mouse 129/SvEvTACBr BAC Library   TCAG
Genomic RPCI-23 Mouse C57BL/6J BAC Library   TCAG
Genomic Screening of library filters (first probe)   TCAG
  Additional probes    
Genomic NIA/NIH 15,000 mouse cDNA clone set unique cDNA clones are derived from 52,374 ESTs from pre- and periimplantation embryos, E12.5 female gonad/mesonephros, and newborn ovary. Average insert size 1.5 kb   TCAG
Genomic NOD Mouse BAC library (end sequenced)   TCAG
Others Ordered mouse ORF set in pMAGIC donor plasmids, sequenced, with no stop codon upstream or downstream (for making constructs). In progress.   Hughes Lab
Gene Trap ES Cell Libraries
To access services below please contact Reno Pontarollo
  CMHD Gene Trap Resource   CMHD
  MICB Gene Trap Resource ES cell clone (www.EScells.ca) available MFGC
  Screening of library filters (first probe)
Additional probes 		available MFGC
Other
To access services below please contact Reno Pontarollo
Recombinant Congenic Lines 22 BcA strains (12.5% C57Bl/6J genome on A/J background) available under MTA, including strain
BcA68, bearing a loss of function mutation in apolipoprotein A1 (ApoA1) gene. 		  Emerillon
Recombinant Congenic Lines 12 AcB strains (12.5% A/J genome on C57Bl/6J background) available under MTA, including strains
AcB55, AcB61 and AcB62, bearing loss of function mutation in the pyruvate kinase (Pklr) gene. 		  Emerillon

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Table B* - Services for Generating Genetically Altered Mice

Service Description Estimated Throughput Source
Transgenesis
To access services below please contact Reno Pontarollo
  TCP transgenic core will generate transgenic mice by pronuclear microinjection into zygotes Service available in 2007 TCP
Pronuclear Injections GMC will provide pronuclear oocyte injections using DNA provided by the user following our protocol. 3 founder transgenic animals will be provided. Service available regionally GMC
ES Cell Chimeras
To access services below please contact Reno Pontarollo
Blastocysts Injections TCP transgenic core will provide blastocyst microinjection service using C57BL/6 host embryos for 129-derived ES cells or BALB/c host embryos for C57BL/6-derived ES cells Service available in 2007 TCP
Blastocysts Injections GMC will provide blastocyst microinjection service using C57BL/6 host embryos for 129-derived ES cells or BALB/c host embryos for C57BL/6-derived ES cells Service available nationally MFGC
Aggregations TCP transgenic core will aggregate F1-hybrid and 129-derived ES cells with outbred ICR embryos to produce chimeras Service available in 2007 TCP
Aggregations GMC will generate chimeras using outbred ICRs as donors of host embryos for aggregations with 129-derived ES cells Service available regionally, early 2005 GMC
Tetraploid Complementation TCP transgenic core will aggregate F1-hybrid ES cells with host tetraploid embryos to produce embryos/mice 100% derived from targeted hybrid ES cells Limited Service now, full service available in 2007 TCP
Tetraploid Complementation GMC will aggregate F1-hybrid ES cells with host tetraploid embryos to produce embryos/mice 100% derived from targeted hybrid ES cells Service available regionally, early 2005 GMC
ENU Screens
To access services below please contact Reno Pontarollo
  CMHD will provide investigators with male F1 offspring from ENU mutagenized mice to carry out ENU screens of their choice within their facility   CMHD
Dominant, Recessive, & Sensitized Screens CMHD will consider proposals from interested investigators to implement new dominant, recessive and/or genetic or pharmacological modifier screens within their ENU program to look for disease models within their area of interest   CMHD

* PLEASE NOTE: The CMC plans to have all technology and services available nationally to the Canadian Scientific Community by 2007. Your current Genome Canada proposal requirements are a priority to us. With sufficient notice, most of these services can be made available to you in 2005 in consultation with the individual CMC resource Director. The listed prices are estimated solely for the purpose of budgeting for Genome Canada Competition III proposals. Prices may vary depending on the scale of your requirements. We anticipate significant reductions in fees should the CMC platforms themselves be funded in future or associated competitions.

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2. Phenotyping

Table C - Services for Phenotyping Genetically Altered Mice
Service Description Estimated Throughput Source
Phenotyping
To access services below please contact Reno Pontarollo
Physiology General behaviour and morphology (per mouse)   CMHD
Physiology X-ray analysis (per sample)   CMHD
Physiology Bone & Soft Tissue Composition Analysis (DEXA) (per sample)   CMHD
Physiology Hematology (per sample)   CMHD
Physiology Blood Biochemistry (per sample)   CMHD
Physiology Blood Collection (per sample)   CMHD
Physiology Blood Pressure & Heart Rate (per 1-4 mice, 3 measurements taken and averaged)   CMHD
Physiology Aortic Blood Velocity (per sample)   CMHD
Physiology Signal Averaged ECG (per sample)   CMHD
Physiology Glucose/Insulin Tolerance (per sample)   CMHD
Physiology Urine Composition (per sample)   CMHD
Physiology PTZ-induced seizures   Emerillon
Physiology Electroshock-induced seizures   Emerillon
Behaviour Morris Water Maze 16 per week Roder Lab Neurobehavioural Core
Behaviour Porsolt Swim Test 10 per day Roder Lab Neurobehavioural Core
Behaviour Open Field Test 10 per day Roder Lab Neurobehavioural Core
Behaviour Grip Strength Test 20 per day Roder Lab Neurobehavioural Core
Behaviour Pre-Pulse Inhibition 20 per 3 days Roder Lab Neurobehavioural Core
Behaviour Acoustic Startle Reactivity 20 per 3 days Roder Lab Neurobehavioural Core
Behaviour Contextual Fear Conditioning 10 per 2 days Roder Lab Neurobehavioural Core
Behaviour Gait Test 10 per day Roder Lab Neurobehavioural Core
Behaviour Latent Inhibition 20 per 8 days Roder Lab Neurobehavioural Core
Behaviour Light/Dark Box 40 per 2 weeks Roder Lab Neurobehavioural Core
Imaging Modalities High-throughput mouse high-field magnetic resonance imaging (MRI)   MICe
Imaging Modalities High-frequency ultrasound biomicroscopes (UBM) for in vivo cardiovascular and embryonic imaging   MICe
Imaging Modalities UBM-guided microinjections   MICe
Imaging Modalities Micro-Computed Tomography (micro-CT)   MICe
Imaging Modalities Optical Projection Tomography Service available late-2006 MICe
Pathology
To access services below please contact Reno Pontarollo
Gross Pathology Whole-body necropsy (no perfusion)   CMHD
  Whole-body necropsy (perfusion)    
  System- or organ-specific necropsy    
  Tissue freezing (snap freezing fresh tissue at -196C per tissue)    
  Necropsy technologist consultation or teaching (per hour)    
Gross Pathology Electron Microscopy    
  Tenai 20 (scope time)   CMHD
  TEM specimen preparation
(Standard fixation, dehydration, embedding, 5 Toluidine blue survey sections on one slide, 2 standard double-stained grids) 		   
  XL30 ESEM (Scope Time)    
  SEM specimen preparation
(Standard fixation, critical point dry, sputter coat)		    
Histology Paraffin processing (per block)
   CMHD
  Embedding only
    
  Decal & embedding only    
  Special preparation    
  Re-embed    
Histology Frozen tissue handling (per block)
 CMHD
  Freezing    
  Cryosectioning    
Histology Sectioning from block (per glass slide)   CMHD
  Paraffin or frozen    
Histology Staining (per glass slide)
   CMHD
  H&E    
  Counter stain only    
  Special stain - simple    
  Special stain - complex    
  Enzymes
    
  Tunel Stain
   
  Immunofluorescence
   
  Immunohistochemistry
   
Histology Histotechnologist consultation or teaching (per hour)   CMHD
Slide Scanning Whole tissue section slide scanning (per glass slide)   CMHD
  20x equivalent on * DVD-R or web access (login required)    
  40x equivalent on * DVD-R or web access (login required)    
  * DVD-R not included    
Laser Capture Microdissection Hourly rate includes staining, images, capture caps, etc.
   CMHD
  DNA extraction (3 isolations)    
  RNA extraction (1 isolation)    
  RNA amplification (1 round)    
  Paradise Kit (paraffin sections for RNA extractions and amplification)    
Pathology Pathologist Consultation (per hour - includes complete gross- and histopathological evaluation with image-enabled electronic report, digital photomicrographs and recommendations)   CMHD
Phenotype Investigation & Characterization Juvenile and Adult (per mouse - includes necropsy, macroimaging, 40-tissue sample collection and single somatic tissue freezing, histological processing of two decalcified and six paraffin blocks, histopathological evaluation with image-enabled electronic report, digital photomicrographs and recommendations)   CMHD
Phenotype Investigation & Characterization Embryonic lethal/Neonatal mortality (per pregnant or post-partum female mouse - includes necropsy of the female mouse, systematic embryo and embryonic tissue collection (including embryo tissue collection for genotyping), morphometry and macroimaging of embryos and extra-embryonic tissue, histological sample processing with histopathological evaluation and image-enabled electronic report including digital photomicrographs and recommendations)   CMHD

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3. Genotyping & Gene Expression Analysis

Table D - Services for Genotyping & Gene Expression of Genetically Altered Mice
Service Description Estimated Throughput Source
Genome Scans
To access services below please contact Reno Pontarollo
Microsatellite Per successful genotyping reaction 50,000 per year Osborne CMHD/TCAG
Microsatellite Radioactive PCR on acrylamide gels   Emerillon
Microsatellite PCR on agarose gels   Emerillon
Microsatellite Marker assisted selection protocol (speed congenics)   Emerillon
SNPs Per successful genotyping reaction Service available early-2005 Osborne CMHD/TCAG
Karyotyping Mouse ES cell karyotyping   TCAG
Karyotyping Mouse SKY karyotyping Service available early-2005 TCAG
FISH Mapping FISH mapping (human or mouse)   TCAG
Microarrays - Gene Expression Chips Affymatrix Mouse 430 2.0   TCAG
Microarrays - Gene Expression Chips Affymatrix Mouse MOE 430 A 2.0   TCAG
Microarrays - Gene Expression Chips Affymatrix Mouse MOE 430 A or B   TCAG
Microarrays - Gene Expression Chips Rat RAE 230 2.0   TCAG
Microarrays - Gene Expression Chips Rat RAE 230 A or B   TCAG
Microarrays - Gene Expression Chips Murine Genome U74 A   TCAG
Microarrays - Gene Expression Chips Rat Genome U34 A   TCAG
Microarrays - Gene Expression Chips Test Array   TCAG
Microarrays - Gene Expression Chips Test Array in combination with any of the above chips   TCAG
Microarrays - Custom Agilent arrays (42K oligonucleotides) 42K known and predicted protein-coding genes   Hughes
Microarrays - Custom Agilent arrays (42K oligonucleotides) All known noncoding RNAs (including miRNAs)   Hughes
SAGE Construction of library using Long (21mer) µSAGE or SAGElite Please enquire CMSGSC
SAGE Sequencing for SAGE (~27-30 tags/read)   CMSGSC
High-Throughput Sequencing Plasmids, PCR products, BAC ends etc.   TCAG
Mouse Molecular Cytogenetics
To access services below please contact Reno Pontarollo
Spectral Karyotyping SKY analysis of 20 metaphase cells per sample of primary or cultured cells. See note GCCRD
FISH FISH analysis of 20 metaphase or interphase cells, in two or three dimensions, with one or two probes See note GCCRD
Comparative Genomic Hybridization CCG analysis for matched control and experimental samples. See note GCCRD
Karyotyping Karyotype analysis for ES cell and cell lines   GCCRD

Note: all technologies in the applicable service are currently available regionally, service for full national service availability is expected late 2005. Prices may be significantly reduced depending on depth of analysis, sample preparation and reagent requirements of the user.

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4. Archiving, Maintenance, Recovery & Distribution

Table E - Services for Archiving, Maintenance, Recovery & Distribution of Genetically Altered Mice

Service Description Estimated Throughput Source
Embryos
To access services below please contact Reno Pontarollo
Embryos Custom Cryo for a homozygous line
 Service available nationally CMMR
  Subsequent freezing session  
  Custom Cryo for a heterozygous line  
  Subsequent freezing session  
  Speed Cryo: fresh IVF and embryo freeze
 
  Subsequent freezing session  
  Basic Cryo: natural mating and embryo freeze
 
  Subsequent freezing session
  Cryo Recovery: thaw embryos and transfer
  Annual storage fee/350-400 embryos
Embryos Custom Cryo for a homozygous line Limited availability, regional service spring 2005 available GMC
  Custom Cryo for a heterozygous line  
  Speed Cryo: fresh IVF and embryo freeze
 
  Basic Cryo: natural mating and embryo freeze
 
  Subsequent Basic Cryo freeze sessions
 
  Cryo Recovery: thaw embryos and transfer  
  Annual storage fee/350 embryos  
Gametes
To access services below please contact Reno Pontarollo
Ovary Cryopreservation Ovary collection and freezing Service available nationally CMMR
Ovary thaw and transplant  
Annual storage fee for 2 ovaries (4 cryovials)  
Ovary Cryopreservation Ovary collection and freezing
 Limited availability, regional service spring 2005 GMC
Ovary thaw and transplant  
Annual storage fee for 2 ovaries (4 cryovials)  
Sperm Cryopreservation Sperm collection and freeze per male Service available nationally CMMR
Re-derivation from frozen sperm  
Annual storage fee, per aliquot  
Sperm Cryopreservation Sperm collection and freeze per male regional service available GMC
Re-derivation from frozen sperm  
Annual storage fee, per aliquot  
Strain Rescue Strain rescue (fresh IVF+embryo transfer) Service available nationally CMMR
1 ICSI experiment  
Strain Rescue Strain rescue (fresh IVF+embryo transfer) Limited availability, regional service spring 2005 GMC
1 ICSI experiment  
Speed Expansion Fresh IVF with ~20 donor females to rapidly expand a colony Service available nationally CMMR
Speed Expansion Fresh IVF with ~20 donor females to rapidly expand a colony Limited availability, regional service spring 2005 GMC

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5. Informatics & Databases

Table F - Services for Bioinformatics and Database Resources of Genetically Altered Mice
Service Description Estimated Throughput Source
Tools for Datamining Statistical inference analysis (e.g. SVM),
large-scale sequence analysis (e.g. BLAT or BLAST mapping),
exploratory analysis on large data sets (e.g. clustering of microarray or SAGE data),
statistical analysis of relationships between gene expression patterns and other information (e.g. Gene Ontology labels),
custom applications 		  Hughes
Databases of Canadian Mouse Resources http://www.EScells.ca   MFGC
Databases of Canadian Mouse Resources http://www.IGTC.ca   MFGC
Databases of Canadian Mouse Resources Canadian Mouse Consortium   MFGC

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